Coding

Part:BBa_K3926004:Design

Designed by: Zhipeng Gao   Group: iGEM21_XHD-Wuhan-A-China   (2021-10-19)


napA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2354
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1202
    Illegal BglII site found at 1346
    Illegal XhoI site found at 1069
    Illegal XhoI site found at 2179
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 46
    Illegal NgoMIV site found at 1296
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1740
    Illegal NgoMIV site found at 2124
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1533
    Illegal BsaI.rc site found at 1492
    Illegal SapI site found at 1797


Design Notes

tga should be cut if you want link it to a indicator protein(eGFP, amilCP, etc.)


Source

Sinorhizobium fredii HH103, sequence found through Genbank: https://www.ncbi.nlm.nih.gov/nuccore/NC_016812.1?report=genbank&from=1015303&to=1017807

References